![AffiAB® Anti-eIF4EBP1 Antibody [JJ09-25]](/web/image/product.product/74141/image_1024/%5BAFG-HB-1313%5D%20AffiAB%C2%AE%20Anti-eIF4EBP1%20Antibody%20%5BJJ09-25%5D%20?unique=a48b62f)
AffiAB® Anti-eIF4EBP1 Antibody [JJ09-25]
This gene encodes one member of a family of translation repressor proteins. The protein directly interacts with eukaryotic translation initiation factor 4E (eIF4E) , which is a limiting component of the multisubunit complex that recruits 40S ribosomal subunits to the 5' end of mRNAs. Interaction of this protein with eIF4E inhibits complex assembly and represses translation. This protein is phosphorylated in response to various signals including UV irradiation and insulin signaling, resulting in its dissociation from eIF4E and activation of cap-dependent mRNA translation.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: Q13541 Human; SwissProt: Q60876 Mouse; SwissProt: Q62622 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JJ09-25
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IF-Cell, IF-Tissue, IHC-P, IP, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide with Human eIF4EBP1 aa 1-49 / 118.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
HIF-1 signaling pathway, PI3K-AKT, 衰老, ErbB signaling pathway, AMPK signaling pathway
Recommended dilutions
WB: 1:1, 000-1:2, 000; IF-Cell: 1:50-1:200; IF-Tissue: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
13 kDa
Subcellular location
Cytoplasm, cytosol.
Positive control
K562 cell lysate, HepG2 cell lysate, 293 cell lysate, Hela, MCF-7, human colon carcinoma tissue, human breast carcinoma tissue, NIH/3T3.