AffiAB® Anti-E2F1 Antibody [JJ092-02]

https://www.affiab.com/web/image/product.template/99958/image_1920?unique=a48b62f
(0 review)

CAT# AFG-HB-1264
Size: 100 μL

496.50 496.5 USD 496.50

496.50

For Price Contact info@affigen.com

This combination does not exist.

Terms and Conditions
30-day money-back guarantee
Shipping: 2-3 Business Days

AffiAB® Anti-E2F1 Antibody [JJ092-02]

The human retinoblastoma gene product appears to play an important role in the negative regulation of cell proliferation. Functional inactivation of Rb can be mediated either through mutation or as a consequence of interaction with DNA tumor virus-encoded proteins. Of all the Rb associations described to date, the identification of a complex between Rb and the transcription factor E2F most directly implicates Rb in regulation of cell proliferation. E2F was originally identified through its role in transcriptional activation of the adenovirus E2 promoter. Sequences homologous to the E2F binding site have been found upstream of a number of genes that encode proteins with putative functions in the G1 and S phases of the cell cycle. E2F-1 is a member of a broader family of transcription regulators including E2F-2, E2F-3, E2F-4, E2F-5, E2F-6 and E2F-7 each of which forms heterodimers with a second protein, DP-1, forming an "active" E2F transcriptional regulatory complex.

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: Q01094 Human; SwissProt: Q61501 Mouse; SwissProt: O09139 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

JJ092-02

KO/KD

N/A

Species reactivity

Human, Mouse

Tested applications

WB, IF-Cell, IF-Tissue, IHC-P, IP, FC

Predicted species reactivity

Rat

Immunogen

Synthetic peptide within Human E2F1 aa 371-404 / 437.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

衰老

Recommended dilutions

WB: 1:1, 000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

Molecular Weight

Predicted band size: 47 kDa

Subcellular location

Nucleus.

Positive control

HepG2 cell lysates, NIH/3T3 cell lysates, Hela, MCF-7, HepG2, mouse pancreas tissue, human breast carcinoma tissue, human tonsil tissue, human colon tissue, human pancreas tissue.