AffiAB® Anti-DOG1 Antibody
ANO1 (anoctamin 1) , also known as DOG1, ORAOV2, TAOS2 or TMEM16A, is a 986 amino acid multi-pass membrane protein that localizes to both the cell membrane and the cytoplasm and belongs to the anoctamin family. Expressed in a variety of tissues with highest expression in liver, gastrointestinal muscle and skeletal muscle, ANO1 functions as a calcium-activated chloride channel that is required for normal tracheal development. Human ANO1 shares 90% sequence identity with its mouse counterpart, suggesting a conserved role between species. ANO1 is present in breast, pancreatic, gastric, and uterine cancers, as well as in neck, ovarian and parathyroid tumors, suggesting a role for ANO1 in carcinogenesis. Three isoforms of ANO1 exist due to alternative splicing events.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q5XXA6 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within C-terminal human CD43.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1mg/mL
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB:1:500-1:1000; IF-Cell:1:50-1:200; IHC-P:1:50-1:200; FC:1:50-1:100
Molecular Weight
114/97 kDa (Predicted band size)
Subcellular location
Cell membrane, cytoplasm.
Positive control
PC-3M cell lysates, A549, HepG2, human liver carcinoma tissue, human seminal pouch tissue.