![AffiAB® Anti-DNA Ligase IV Antibody [JM64-32]](/web/image/product.product/74037/image_1024/%5BAFG-HB-1199%5D%20AffiAB%C2%AE%20Anti-DNA%20Ligase%20IV%20Antibody%20%5BJM64-32%5D%20?unique=a48b62f)
AffiAB® Anti-DNA Ligase IV Antibody [JM64-32]
The X-ray repair cross-complementing protein XRCC4 and DNA Ligase IV are essential for repairing double-strand breaks in DNA. These stories form a critical complex composed of two molecules of each protein that preferentially bind DNA with nicks or broken ends. As an obligatory accessory molecule, XRCC4 binds to DNA Ligase IV and enhances its joining activity. The XRCC4 / DNA Ligase IV complex is also involved in V (D) J recombination. V (D) (in) a pair of high incidence of apoptosis in the development nervous system and a block in B and T cell maturation.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P49917 Human
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JM64-32
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IHC-P, IF-Cell
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human DNA Ligase IV aa 484-705 / 911.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:2, 000; IHC-P: 1:50-1:200; IF-Cell: 1:10-1:50
Molecular Weight
Predicted band size: 104 kDa
Subcellular location
Nucleus.
Positive control
HepG2 cell lysate, Hela cell lysate, Jurkat cell lysate, human colon carcinoma tissue, human placenta tissue.