AffiAB® Anti-DLL4 Antibody
The LIN-12/Notch family of transmembrane receptors is believed to play a central role in development by regulating cell fate decisions. Notch proteins have been found to be overexpressed or rearranged in human tumors. Ligands for Notch include Jagged, Jagged-2 and Delta. While blocking the differentiation of progenitor cells into the B-cell lineage, Delta promotes the emergence of a population of cells with T cell/NK-cell characteristics. The protein is a membrane protein expressed in heart, pancreas, brain and muscle during gastrulation and early organogenesis and in adult heart and lung. Delta-4 is a membrane protein that activates Notch-1 and Notch-4. It is expressed in a wide range of adult and fetal tissues, especially in vascular endothelium.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q9NR61 Human; SwissProt: Q9JI71 Mouse; SwissProt: D3ZHH1 Rat
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human DLL4 aa 175-370 / 685.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Notch signaling pathway
Recommended dilutions
WB: 1:500-1:1, 000; IF-Cell: 1:50-1:200; IHC-P: 1:50-1:200; FC: 1:50-1:100
Molecular Weight
74 kDa (Predicted band size)
Subcellular location
Cell membrane.
Positive control
Mouse placenta tissue lysate, HUVEC cell lysate, 293T, HUVEC, PMVEC, human liver tissue, human kidney tissue.