![AffiAB® Anti-DDAH2 Antibody [JB59-33]](/web/image/product.product/73992/image_1024/%5BAFG-HB-1148%5D%20AffiAB%C2%AE%20Anti-DDAH2%20Antibody%20%5BJB59-33%5D%20?unique=a48b62f)
AffiAB® Anti-DDAH2 Antibody [JB59-33]
DDAH, a dimethylarginine dimethylaminohydrolase, hydrolyzes dimethyl arginine (ADMA) and monomethyl arginine (MMA) , both inhibitors of nitric oxide synthases, and may be involved in in-vivo modulation of nitric oxide production. Impairment of DDAH causes ADMA accumulation and a reduction in cGMP generation. DDAH II, the predominant DDAH isoform in endothelial cells, facilitates the induction of nitric oxide synthesis by all-trans-Retinoic acid (atRA) . DDAH proteins are highly expressed in colon, kidney, stomach and liver tissues.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: O95865 Human; SwissProt: Q99LD8 Mouse; SwissProt: Q6MG60 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
JB59-33
KO/KD
N/A
Species reactivity
Human, Mouse, Rat
Tested applications
WB, IP
Predicted species reactivity
N/A
Immunogen
Recombinant protein within Human DDAH2 aa 1-125 / 285.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:2, 000-1:5, 000
; IP: 1:10-:50
Molecular Weight
Predicted band size: 30 kDa
Subcellular location
Cytoplasm. Mitochondrion.
Positive control
Rat heart tissue lysate, mouse heart tissue lysate, mouse lung tissue lysate, human lung tissue lysate.