AffiAB® Anti-D14 Antibody
Involved in strigolactone (SL) signaling pathway. May function downstream of SL synthesis, as a component of hormone signaling or as an enzyme that participates in the conversion of SL to the bioactive form. Strigolactones are hormones that inhibit tillering and shoot branching through the MAX-dependent pathway, contribute to the regulation of shoot architectural response to phosphate-limiting conditions and function as rhizosphere signal that stimulates hyphal branching of arbuscular mycorrhizal fungi and trigger seed germination of root parasitic weeds. Strigolactone-dependent association of D14 with D3 and D53 (a repressor of SL signaling) triggers D53 ubiquitination and degradation . Hydrolyzes the butenolide ring of SLs . A reaction product D-OH is trapped in the cavity of D14, inducing the interaction with SLR1, and probably with other proteins such as D3 and D53 . Contributes to the negative regulation of gibberellin signaling .
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: Q10QA5
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
N/A
Species reactivity
Rice
Tested applications
WB, IHC-P, IF-Tissue
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within N-terminal Rice D14.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/ml.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500-1:1000; IHC-P: 1:50-1:200; IF-Tissue: 1:50-1:200
Molecular Weight
Predicted band size 33 kDa.
Subcellular location
Cytoplasm, Nucleus.
Positive control
Rice.