AffiAB® Anti-Cytokeratin 7 Antibody - Cytoskeleton Marker
Keratin-7 is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. Keratin-7 is found in simple glandular epithelia, and in transitional epithelium. Epithelial cells of the lung and breast both contain keratin-7, but some other glandular epithelia, such as those of the colon and prostate, do not. Because the keratin-7 antigen is found in both healthy and neoplastic cells, antibodies to CK7 can be used in immunohistochemistry to distinguish ovarian and transitional cell carcinomas from colonic and prostate cancers, respectively.
Antibody type
Rabbit polyclonal Antibody
Uniprot ID
SwissProt: P08729 Human
Recombinant
NO
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
N/A
KO/KD
Knockdown validated
Species reactivity
Human
Tested applications
WB, IF-Cell, IHC-P, FC
Predicted species reactivity
N/A
Immunogen
Synthetic peptide within Human CytokeRatin 7 aa 420-469 / 469.
Storage
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*PBS (pH7.4) , 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Immunogen affinity purified.
Signal pathway
N/A
Recommended dilutions
WB: 1:500; IF-Cell: 1:200; IHC-P: 1:200; FC: 1:50-1:100
Molecular Weight
Predicted band size: 51 kDa
Subcellular location
Cytoplasm, Intermediate filament, Keratin.
Positive control
Wild-type Hela whole cell lysate, A549 cell lysate, Hela, MCF-7, SW480, human breast carcinoma tissue, human ovary carcinoma tissue.