![AffiAB® Anti-14-3-3 Theta Antibody [SD084-02]](/web/image/product.product/66159/image_1024/%5BAFG-HB-0009%5D%20AffiAB%C2%AE%20Anti-14-3-3%20Theta%20Antibody%20%5BSD084-02%5D%20?unique=a48b62f)
AffiAB® Anti-14-3-3 Theta Antibody [SD084-02]
14-3-3 protein theta is a protein that in humans is encoded by the YWHAQ gene. This gene product belongs to the 14-3-3 family of proteins that mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals, and this protein is 99% identical to the mouse and rat orthologs. This gene is upregulated in patients with amyotrophic lateral sclerosis. It contains in its 5' UTR a 6 bp tandem repeat sequence that is polymorphic; however, there is no correlation between the repeat number and the disease.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P27348 Human; SwissProt: P68254 Mouse; SwissProt: P68255 Rat
Recombinant
VRAI
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SD084-02
KO/KD
N/A
Species reactivity
Human
Tested applications
WB, IF-Cell, IF-Tissue, FC
Predicted species reactivity
Mouse, Rat
Immunogen
Synthetic peptide within Human 14-3-3 Theta aa 1-50 / 245.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Neuroscience, PI3K-AKT
Recommended dilutions
WB: 1:500-1:5, 000; IF-Cell: 1:100-1:500; IF-Tissue: 1:100-1:500; FC: 1:50-1:100
Molecular Weight
Predicted band size: 28 kDa
Subcellular location
Cytoplasm.
Positive control
MCF-7 cell lysate, Hela cell lysate, Hela, A431, A549, SH-SY5Y.