AffiAB® Anti-14-3-3 alpha+beta Antibody [SD0837]
14-3-3 protein beta/alpha is a protein that in humans is encoded by the YWHAB gene. This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
Antibody type
Recombinant Rabbit monoclonal Antibody
Uniprot ID
SwissProt: P31946 Human; SwissProt: Q9CQV8 Mouse; SwissProt: P35213 Rat
Recombinant
YES
Conjugation
Non-conjugated
Host
Rabbit
Isotype
IgG
Clone
SD0837
KO/KD
N/A
Species reactivity
Human, Mouse
Tested applications
WB, IF-Cell, IHC-P, FC, IP
Predicted species reactivity
Rat
Immunogen
Synthetic peptide corresponding to C terminal Human 14-3-3 alpha + beta aa 197-246 / 246.
Storage
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Form
Liquid
Storage buffer
1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1 mg/mL.
Purity
Protein A affinity purified.
Signal pathway
Neuroscience, PI3K-AKT
Recommended dilutions
WB: 1:500-1:2, 000; IF-Cell: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.
Molecular Weight
Predicted band size: 28 kDa
Subcellular location
Cytoplasm, Melanosome.
Positive control
Hela cell lysate, HepG2 cell lysate, 293T cell lysate, SK-Br-3 cell lysate, Hela, A431, SHG-44, human breast carcinoma tissue, mouse brain tissue, mouse skin tissue, human breast tissue.