AffiAB® Anti-14-3-3 alpha+beta Antibody [SD0837]

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CAT# AFG-HB-0001
Size: 100 µL

433.50 433.5 USD 433.50

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AffiAB® Anti-14-3-3 alpha+beta Antibody [SD0837]

14-3-3 protein beta/alpha is a protein that in humans is encoded by the YWHAB gene. This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.​

Antibody type

Recombinant Rabbit monoclonal Antibody

Uniprot ID

SwissProt: P31946 Human; SwissProt: Q9CQV8 Mouse; SwissProt: P35213 Rat

Recombinant

YES

Conjugation

Non-conjugated

Host

Rabbit

Isotype

IgG

Clone

SD0837

KO/KD

N/A

Species reactivity

Human, Mouse

Tested applications

WB, IF-Cell, IHC-P, FC, IP

Predicted species reactivity

Rat

Immunogen

Synthetic peptide corresponding to C terminal Human 14-3-3 alpha + beta aa 197-246 / 246.

Storage

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Form

Liquid

Storage buffer

1*TBS (pH7.4) , 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Concentration

1 mg/mL.

Purity

Protein A affinity purified.

Signal pathway

Neuroscience, PI3K-AKT

Recommended dilutions

WB: 1:500-1:2, 000; IF-Cell: 1:100-1:500; IHC-P: 1:50-1:200; FC: 1:50-1:100; IP: Use at an assay dependent concentration.

Molecular Weight

Predicted band size: 28 kDa

Subcellular location

Cytoplasm, Melanosome.

Positive control

Hela cell lysate, HepG2 cell lysate, 293T cell lysate, SK-Br-3 cell lysate, Hela, A431, SHG-44, human breast carcinoma tissue, mouse brain tissue, mouse skin tissue, human breast tissue.