![AffiAB® Anti-Hu CD14 PE-Cy5 [MEM-15]](/web/image/product.product/83749/image_1024/%5BAFG-EXB-2250%5D%20AffiAB%C2%AE%20Anti-Hu%20CD14%20PE-Cy5%20%5BMEM-15%5D?unique=d31c23b)
Overview
CD14 is a 55 kDa GPI-anchored glycoprotein, constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils, where it serves as a multifunctional lipopolysaccharide receptor. It is also released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 is able to discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. CD14 affects allergic, inflammatory and infectious processes.
Specificity:
The antibody MEM-15 reacts with CD14, a 53-55 kDa GPI (glycosylphosphatidylinositol) -linked extracellular membrane glycoprotein expressed on monocytes, macrophages and weakly on granulocytes; also expressed by most tissue macrophages. The antibody MEM-15 also reacts with soluble forms of CD14 found in serum and in the urine of some nephrotic patients.
Antigen
CD14
Clone
MEM-15
Species Reactivity
Non-human primates, Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
A crude mixture of human urinary proteins precipitated by ammonium sulphate from the urine of a patient suffering from proteinuria.
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PE-Cy5
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide