![AffiAB® Anti-Ki-67 PE-Cy7 [Ki-67]](/web/image/product.product/83655/image_1024/%5BAFG-EXB-2156%5D%20AffiAB%C2%AE%20Anti-Ki-67%20PE-Cy7%20%5BKi-67%5D?unique=0abe440)
Overview
Ki-67 is a highly protease-sensitive nuclear protein expressed in two isoforms (345 kDa and 395 kDa), both of which are identified by the antibody clone Ki-67. The Ki-67 antigen is essential for cell proliferation and its expression is restricted to the cycling cells. It is detected in G1, S, G2 and M phase, whereas it is absent in cells which are in G0 phase and it is not associated with DNA repair processes. Ki-67 thus represents an important tool for detection of proliferating cells, which is of great importance in tumor diagnostics and is commonly used as a prognostic factor in cancer studies.
Specificity:
The mouse monoclonal antibody Ki-67 recognizes Ki-67 antigen, a non-histone nuclear protein expressed exclusively in proliferating cells.
Antigen
Ki-67
Clone
Ki-67
Species Reactivity
Cow, Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Nuclei of the Hodgkin lymphoma cell line L428
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PE-Cy7
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide