![AffiAB® Anti-Hu CD95 PE-DyLight 594 [LT95]](/web/image/product.product/83642/image_1024/%5BAFG-EXB-2143%5D%20AffiAB%C2%AE%20Anti-Hu%20CD95%20PE-DyLight%20594%20%5BLT95%5D?unique=d31c23b)
Overview
CD95 (Fas, APO-1), a 46 kDa transmembrane glycoprotein, is a cell death receptor of the TNFR superfamily. Stimulation of CD95 results in aggregation of its intracellular death domains, formation of the death-inducing signaling complex (DISC) and activation of caspases. In type I cells caspase 3 is activated by high amounts of caspase 8 generated at the DISC, in type II cells low concentration of caspase 8 activates pathway leading to the release of cytochrome c from mitochondria and activation of caspase 3 by cytochom c. Besides its roles in induction of apoptosis, Fas also triggers pro-inflammatory cytokine responses.
Specificity:
The antibody LT95 reacts with an extracellular epitope on CD95 (Fas/APO-1), a 46 kDa single chain type I glycoprotein of the tumour necrosis factor/nerve growth factor (TNF/NGF) receptor superfamily, expressed on a variety of normal and neoplastic cells. It seems that the antibody LT95 does not induce Fas mediated apoptosis, although it cross-blocks anti-Fas DX2 antibody that recognizes a functional epitope of Fas molecule.
Antigen
CD95
Clone
LT95
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
HUT-78 human T cell lymphoma cell line
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PE-DyLight® 594
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide