![AffiAB® Anti-Hu CD16 PerCP [3G8]](/web/image/product.product/83554/image_1024/%5BAFG-EXB-2055%5D%20AffiAB%C2%AE%20Anti-Hu%20CD16%20PerCP%20%5B3G8%5D?unique=d31c23b)
Overview
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Specificity:
The mouse monoclonal antibody 3G8 recognizes an extracellular epitope of CD16, a low affinity receptor for aggregated IgG (FcgammaRIII antigen) . CD16 exists in two different isoforms: CD16a (FcgammaRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcgammaRIIIB; 48 kDa; mainly expressed on neutrophils) . Regarding CD16-158V/F polymorphism, the antibody 3G8 detects both 158V and 158F allotype on natural NK cells.
Antigen
CD16
Clone
3G8
Species Reactivity
Non-human primates, Human
Negative Species
N/A
Isotype
Mouse IgG1 kappa
Immunogen
Human neutrophils
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PerCP
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide