![AffiAB® Anti-Cyclin D1 Alexa Fluor 647 [DCS-6]](/web/image/product.product/83335/image_1024/%5BAFG-EXB-1836%5D%20AffiAB%C2%AE%20Anti-Cyclin%20D1%20Alexa%20Fluor%20647%20%5BDCS-6%5D?unique=24195c2)
Overview
Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.
Specificity:
The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.
Antigen
Cyclin D1
Clone
DCS-6
Species Reactivity
Rat, Mouse, Non-human primates, Human
Negative Species
Cat, Sheep
Isotype
Mouse IgG2a
Immunogen
recombinant human cyclin D1 (amino acids 1-295)
Application
WB, ICC, FC (QC tested)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Alexa Fluor® 647
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide