![AffiAB® Anti-HLA-ABCE Alexa Fluor 488 [TP25.99SF]](/web/image/product.product/83324/image_1024/%5BAFG-EXB-1825%5D%20AffiAB%C2%AE%20Anti-HLA-ABCE%20Alexa%20Fluor%20488%20%5BTP25.99SF%5D?unique=24195c2)
Overview
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Classical human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules, the non-classical by e.g. HLA-E, HLA-G.
Specificity:
The mouse monoclonal antibody TP25.99SF recognizes an extracellular epitope on HLA-ABC and HLA-E molecules, but not HLA-G. It can be used for discrimination between HLA-G and other HLA-class I antigens.
Antigen
HLA-ABCE
Clone
TP25.99SF
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
IFN-gamma-treated human melanoma cells Colo 38
Application
FC (QC tested), WB
Regulatory status
RUO
Concentration
0.1 mg/ml
Format
Alexa Fluor® 488
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide