![AffiAB® Anti-Hu CD63 Alexa Fluor 488 [MEM-259]](/web/image/product.product/83284/image_1024/%5BAFG-EXB-1785%5D%20AffiAB%C2%AE%20Anti-Hu%20CD63%20Alexa%20Fluor%20488%20%5BMEM-259%5D?unique=d31c23b)
Overview
CD63 (LAMP-3, lysosome-associated membrane protein-3), a glycoprotein of tetraspanin family, is present in late endosomes, lysosomes and secretory vesicles of various cell types. It is also present in the plasma membrane, usually following cell activation. Hence, it has become an widely used basophil activation marker. In mast cells, however, CD63 exposition does not need their activation. CD63 interacts with integrins and affects phagocytosis and cell migration, it is also involved in H/K-ATPase trafficking regulation of ROMK1 channels. CD63 also serves as a T-cell costimulation molecule. Expression of CD63 can be used for predicting the prognosis in earlier stages of carcinomas.
Specificity:
The antibody MEM-259 reacts with an extracellular/luminal epitope of CD63 (LAMP-3), a 40-60 kDa tetraspan glycoprotein expressed by granulocytes, platelets, T cells, monocytes/macrophages and endothelial cells. Cell surface exposition of CD63 is usually activation-dependent.
Antigen
CD63
Clone
MEM-259
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
HPB-ALL T cell line
Application
FC (QC tested), ICC
Regulatory status
RUO
Concentration
N/A
Format
Alexa Fluor® 488
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide