![AffiAB® Anti-Hu CD72 Alexa Fluor 488 [3F3]](/web/image/product.product/83283/image_1024/%5BAFG-EXB-1784%5D%20AffiAB%C2%AE%20Anti-Hu%20CD72%20Alexa%20Fluor%20488%20%5B3F3%5D?unique=d31c23b)
Overview
CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
Specificity:
The mouse monoclonal antibody 3F3 recognizes an extracellular epitope of CD72, a 39-43 kDa type II membrane glycoprotein (C-type lectin family) . CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
Antigen
CD72
Clone
3F3
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG2b
Immunogen
Normal human lymphocytes from a lymph node.
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
Alexa Fluor® 488
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide