![AffiAB® Anti-Hu CD50 Alexa Fluor 488 [MEM-171]](/web/image/product.product/83277/image_1024/%5BAFG-EXB-1778%5D%20AffiAB%C2%AE%20Anti-Hu%20CD50%20Alexa%20Fluor%20488%20%5BMEM-171%5D?unique=d31c23b)
Overview
CD50 (intracellular adhesion molecule 3, ICAM-3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA) -1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (dendritic cell-specific ICAM-3-grabbing nonintegrin, DC-SIGN), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
Specificity:
The antibody MEM-171 recognizes an extracellular epitope in the D2 domain of CD50 (ICAM-3), a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.
Antigen
CD50
Clone
MEM-171
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Human granulocytes
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
Alexa Fluor® 488
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide