![AffiAB® Anti-Hu CD45 Alexa Fluor 488 [MEM-28]](/web/image/product.product/83274/image_1024/%5BAFG-EXB-1775%5D%20AffiAB%C2%AE%20Anti-Hu%20CD45%20Alexa%20Fluor%20488%20%5BMEM-28%5D?unique=d31c23b)
Overview
CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
Specificity:
The antibody MEM-28 reacts with an extracellular epitope on all alternative forms of human CD45 antigen (Leukocyte Common Antigen), a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except erythrocytes and platelets.
Antigen
CD45
Clone
MEM-28
Species Reactivity
Human
Negative Species
Horse
Isotype
Mouse IgG1
Immunogen
Human thymocytes and T lymphocytes.
Application
FC (QC tested), WB, ICC
Regulatory status
RUO
Concentration
N/A
Format
Alexa Fluor® 488
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide