![AffiAB® Anti-Hu CD32 PE [3D3]](/web/image/product.product/83135/image_1024/%5BAFG-EXB-1636%5D%20AffiAB%C2%AE%20Anti-Hu%20CD32%20PE%20%5B3D3%5D?unique=d31c23b)
Overview
CD32 (FcgammaRII) is a low affinity receptor for aggregated IgG. It is strongly expressed on monocytes, granulocytes, myeloid and myeloblastic cell lines, and weakly on B cells, CD34+ bone marrow cells, and resting and activated platelets. After binding its ligand, CD32 induces IgG-mediated phagocytosis and oxidative burst in monocytes and neutrophils, whereas in B cells it mediates a negative signal. This polymorphic transmembrane glycoprotein is expressed not only in the activating (CD32a) and inhibitory isoform (CD32b), but also in individual variants with differing avidities for IgG subtypes (e.g. the CD32a131R and CD32a131H allotypes) .
Specificity:
The mouse monoclonal antibody 3D3 recognizes an extracellular epitope of CD32, a 40 kDa polymorphic transmembrane glycoprotein serving as the low affinity receptor for aggregated IgG. This antibody recognizes CD32 isoforms on B cells of all donors, but on platelets, monocytes, and granulocytes of only some donors (131R variant, but not 131H variant) .
Antigen
CD32
Clone
3D3
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1 kappa
Immunogen
purified glycosylated recombinant human FcgammaRIIa2
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PE
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide