![AffiAB® Anti-Hu CD52 PE [HI186]](/web/image/product.product/82974/image_1024/%5BAFG-EXB-1475%5D%20AffiAB%C2%AE%20Anti-Hu%20CD52%20PE%20%5BHI186%5D?unique=d31c23b)
Overview
CD52 (CAMPATH-1, HE5) is a highly glycosylated GPI-anchored 21-28 kDa glycopeptide which is present at high levels on lymphocytes, macrophages, epithelial cells of male reproductive tract and mature sperm. Its 12-amino acid beckbone carries a complex N-linked carbohydrate moiety, which differs between sperm and leukocyte CD52, as well as the GPI anchor does. CD52 can be acquired by sperm cells from seminal plasma, where it is released by epithelial cells. Although CD52 is not an essential T-cell costimulator, its triggering results in activation of normal human T cells. CD52 is a very good target for antibody/complement-mediated cell lysis.
Specificity:
The antibody HI186 reacts with CD52 (CAMPATH-1), a 21-28 kDa extracellular glycoprotein containing a large N-linked carbohydrate moiety; mature CD52 molecule is actually much smaller (approx. 8-9 kDa) . CD52 is expressed at high levels on lymphocytes, monocytes/macrophages and in male reproductive tract.
Antigen
CD52
Clone
HI186
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG2b
Immunogen
Human tonsil
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PE
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide