![AffiAB® Anti-SIT PE [SIT-01]](/web/image/product.product/82968/image_1024/%5BAFG-EXB-1469%5D%20AffiAB%C2%AE%20Anti-SIT%20PE%20%5BSIT-01%5D?unique=0daa593)
Overview
SIT (SHP2-interacting transmembrane adaptor protein) is expressed exclusively in lymphoid organs and acts either as a positive or as a negative regulatory element in T cell activation and in T cell development. Binding to Grb2 plays a pivotal role in signal transduction. Hubener et al. (2001) determined that the SIT gene contains 5 exons and spans 1.8 kb of genomic DNA. The SIT promoter demonstrated strong transcriptional activity and potential binding sites for both ubiquitous and lymphoid-specific transcription factors.
Specificity:
The antibody SIT-01 reacts with an intracellular epitope of SHP2-interacting transmembrane adaptor protein (SIT) expressed exclusively in lymphoid organs. It weakly crossreacts with murine SIT.
Antigen
SIT
Clone
SIT-01
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Bacterially produced recombinant intracellular fragment of human SIT.
Application
FC (QC tested)
Regulatory status
RUO
Concentration
0.1 mg/ml
Format
PE
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide