![AffiAB® Anti-Hu CD34 PE [4H11[APG]]](/web/image/product.product/82949/image_1024/%5BAFG-EXB-1450%5D%20AffiAB%C2%AE%20Anti-Hu%20CD34%20PE%20%5B4H11%5BAPG%5D%5D?unique=d31c23b)
Overview
CD34 is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.
Specificity:
The mouse monoclonal antibody 4H11[APG] reacts with extracellular class III epitope on CD34, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody 4H11[APG] completely blocks binding of class III antibodies BIRMA K3 and 8G12 on KG1a cell line.
Antigen
CD34
Clone
4H11[APG]
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Permanent human cell line derived from peripheral leucocytes of a patient suffering from chronic myeloid leukaemia.
Application
FC (QC tested), ICC
Regulatory status
RUO
Concentration
N/A
Format
PE
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide