![AffiAB® Anti-PCNA PE [PC10]](/web/image/product.product/82900/image_1024/%5BAFG-EXB-1401%5D%20AffiAB%C2%AE%20Anti-PCNA%20PE%20%5BPC10%5D?unique=0daa593)
Overview
PCNA (proliferating cell nuclear antigen) is the DNA polymerase delta auxiliary protein acting in homotrimeric form to increase the processivity of leading strand synthesis during DNA replication. PCNA is expressed in the nucleus of all proliferating cells. In response to DNA damage, it is ubiquitinated and is involved in the RAD6-dependent DNA repair pathway. PCNA is a useful marker of DNA synthesis, as its form not involved in DNA synthesis degradates in histological preparations in the presence of organic solvents.
Specificity:
The mouse monoclonal antibody PC10 (also known as 3F81) recognizes PCNA, a 36 kDa conserved nuclear protein serving as a cofactor for DNA synthesis.
Antigen
PCNA
Clone
PC10
Species Reactivity
Mouse, Human, Non-human primates, Drosophila, Chicken, Rat
Negative Species
N/A
Isotype
Mouse IgG2a kappa
Immunogen
recombinant rat PCNA
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PE
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide