![AffiAB® Anti-Hu CD11b (activation epitope) PE [CBRM1/5]](/web/image/product.product/82893/image_1024/%5BAFG-EXB-1394%5D%20AffiAB%C2%AE%20Anti-Hu%20CD11b%20%28activation%20epitope%29%20PE%20%5BCBRM1-5%5D?unique=d31c23b)
Overview
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) ; expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Specificity:
The mouse monoclonal antibody CBRM1/5 recognizes an activation-dependent epitope on extracellular part of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells. The antibody recognizes a subset of CD11b molecules on neutrophils and monocytes activated with chemoattractants or phorbol esters and it does not recognize CD11b on non-activated cells.
Antigen
CD11b (activation epitope)
Clone
CBRM1/5
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1 kappa
Immunogen
Information not available
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
PE
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide