![AffiAB® Anti-Ms CD49e FITC [5H10-27 (MFR5) ]](/web/image/product.product/82823/image_1024/%5BAFG-EXB-1324%5D%20AffiAB%C2%AE%20Anti-Ms%20CD49e%20FITC%20%5B5H10-27%20%28MFR5%29%20%5D?unique=d31c23b)
Overview
CD49e, the alpha 5 integrin, noncovalently associates with the beta 1integrin (CD29) to form VLA-5 integrin complex. CD49e itself is composed of two disulfide-linked chains of 135 kDa and 25 kDa. VLA-5 binds to RGD sequence of fibronectin, and to neural adhesion molecule L1. It is important in maintaining the integrity of endothelial monolayers, in monocyte migration into the extracellular tissues, and it also provides a co-stimulatory signal to T cells and enhances receptor and complement receptor-mediated phagocytosis.
Specificity:
The rat monoclonal antibody 5H10-27 (MFR5) recognizes an extracellular epitope of murine CD49e, a 135 kDa protein serving as VLA-5 alpha chain, expressed on thymocytes, activated T cells, splenic B cells, and mast cells.
Antigen
CD49e
Clone
5H10-27 (MFR5)
Species Reactivity
Mouse
Negative Species
N/A
Isotype
Rat IgG2a kappa
Immunogen
C57BL/6 x A/J) F1 murine mast cell line
Application
FC (QC tested)
Regulatory status
RUO
Concentration
0.5 mg/ml
Format
FITC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide