![AffiAB® Anti-Rt CD8b FITC [341]](/web/image/product.product/82791/image_1024/%5BAFG-EXB-1292%5D%20AffiAB%C2%AE%20Anti-Rt%20CD8b%20FITC%20%5B341%5D?unique=d31c23b)
Overview
The CD8b (CD8 beta) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages, mast cells, and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Specificity:
The mouse monoclonal antibody 341 (also known as 34.1) recognizes rat CD8b, the 32-34 kDa beta chain of the CD8 coreceptor (extracellular epitope), expressed on T cell subsets and some other cell types, such as macrophages.
Antigen
CD8b
Clone
341
Species Reactivity
Rat
Negative Species
N/A
Isotype
Mouse IgG1 kappa
Immunogen
CD8 positive Wistar rat splenic T cell hybridomas
Application
FC (QC tested)
Regulatory status
RUO
Concentration
0.5 mg/ml
Format
FITC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide