![AffiAB® Anti-Hu CD64 FITC [10.1]](/web/image/product.product/82783/image_1024/%5BAFG-EXB-1284%5D%20AffiAB%C2%AE%20Anti-Hu%20CD64%20FITC%20%5B10.1%5D?unique=d31c23b)
Overview
CD64 (FcgammaRI) is a cell surface receptor for Fc region of IgG. It is composed of specific ligand binding alpha subunit and promiscuous gamma subunit, which is indispensable for tyrosine-based signaling. However, even the alpha subunit can transduce signals leading to cellular effector functions. The isoform FcgammaRIa1 binds human IgG with high affinity, has limited myeloid cell distribution, and a relatively large intracellular domain. Products of related genes include FcgammaRIb and FcgammaRIc isoforms, but these specify low affinity IgG receptors if functionally expressed at all. Besides a role in antigen clearance, FcgammaRI (a1) can potently enhance MHC class I and II antigen presentation in vitro and in vivo.
Specificity:
The mouse monoclonal antibody 10.1 recognizes an extracellular epitope on CD64/FcgammaRI, a 72 kDa single chain type I glycoprotein, that is expressed on monocytes/macrophages, dendritic cells, and activated granulocytes. The epitope is sensitive to formalin fixation.
Antigen
CD64
Clone
10.1
Species Reactivity
Human, Non-human primates
Negative Species
N/A
Isotype
Mouse IgG1 kappa
Immunogen
Rheumatoid synovial fluid cells and fibronectin purified human monocytes
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
FITC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide