![AffiAB® Anti-Ms CD54 FITC [YN1/1.7.4]](/web/image/product.product/82775/image_1024/%5BAFG-EXB-1276%5D%20AffiAB%C2%AE%20Anti-Ms%20CD54%20FITC%20%5BYN1-1.7.4%5D?unique=d31c23b)
Overview
CD54 (ICAM-1) is a member of the C2 subset of immunoglobulin superfamily. It is a transmembrane molecule with 7 potential N-glycosylated sites, expressed on resting monocytes and endothelial cells and can be upregulated on many other cells, e.g. with lymphokines, on B- and T-lymphocytes, thymocytes, dendritic cells and also on keratinocytes, chondrocytes, as well as epithelial cells. CD54 mediates cell adhesion by binding to integrins CD11a/CD18 (LFA-1) and to CD11b/CD18 (Mac-1) . The interaction of CD54 with LFA-1 enhances antigen-specific T-cell activation.
Specificity:
The rat monoclonal antibody YN1/1.7.4 reacts with an extracellular epitope of CD54 (ICAM-1), a 85-110 kDa type I transmembrane glycoprotein expressed on activated endothelial cells, T lymphocytes, B lymphocytes, monocytes, macrophages, granulocytes and dendritic cells; the expression of CD54 is upregulated by activation.
Antigen
CD54
Clone
YN1/1.7.4
Species Reactivity
Mouse
Negative Species
N/A
Isotype
Rat IgG2b
Immunogen
Mouse NS-1 cells
Application
FC (QC tested)
Regulatory status
RUO
Concentration
0.5 mg/ml
Format
FITC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide