![AffiAB® Anti-CD3 epsilon (activation epitope) FITC [APA1/1]](/web/image/product.product/82766/image_1024/%5BAFG-EXB-1267%5D%20AffiAB%C2%AE%20Anti-CD3%20epsilon%20%28activation%20epitope%29%20FITC%20%5BAPA1-1%5D?unique=d31c23b)
Overview
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation.
Specificity:
The mouse monoclonal antibody APA1/1 recognizes an activation-dependent intracellular epitope of CD3 epsilon. Exposure of the epitope precedes CD3 phosphorylation and recruitment and activation of ZAP70, which initiates the signaling cascade produced by T-cell activation. APA1/1 provides the earliest known marker for TCR-mediated T cell activation.
Antigen
CD3 epsilon (activation epitope)
Clone
APA1/1
Species Reactivity
Mouse, Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Purified human CD3 proteins isolated from thymus
Application
FC (QC tested)
Regulatory status
RUO
Concentration
0.1 mg/ml
Format
FITC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide