![AffiAB® Anti-Ms CD8a FITC [53-6.7]](/web/image/product.product/82754/image_1024/%5BAFG-EXB-1255%5D%20AffiAB%C2%AE%20Anti-Ms%20CD8a%20FITC%20%5B53-6.7%5D?unique=d31c23b)
Overview
The CD8a (CD8 alpha) subunit of CD8 T cell coreceptor is expressed in CD8 alpha/beta heterodimers on majority of MHC I-restricted conventional T cells and thymocytes and in CD8 alpha/alpha homodimers on subsets of memory T cells, intraepithelial lymphocytes, NK cells, macrophages and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha/beta but not alpha/alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Specificity:
The rat monoclonal antibody 53-6.7 recognizes an extracellular epitope of murine CD8a (32-34 kDa; alpha chain of the CD8 antigen) .
Antigen
CD8a
Clone
53-6.7
Species Reactivity
Mouse
Negative Species
N/A
Isotype
Rat IgG2a kappa
Immunogen
Murine spleen cells
Application
FC (QC tested)
Regulatory status
RUO
Concentration
0.5 mg/ml
Format
FITC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide