![AffiAB® Anti-VCP FITC [Hs-14]](/web/image/product.product/82693/image_1024/%5BAFG-EXB-1194%5D%20AffiAB%C2%AE%20Anti-VCP%20FITC%20%5BHs-14%5D?unique=0daa593)
Overview
VCP (valosin-containing protein), also known as p97, TERA, ALS14, IBMPFD, HEL-220, IBMPFD1, or HEL-S-70, is a member of a protein family that includes putative ATP-binding proteins involved in vesicle transport and fusion, 26S proteasome function, and assembly of peroxisomes. VCP is a structural protein that associates with clathrin and heat-shock protein Hsc70, to form a complex. It has been implicated in a number of cellular events that are regulated during mitosis, including homotypic membrane fusion, spindle pole body function, and ubiquitin-dependent protein degradation. In sperm this intra-acrosomal protein can be used as a marker for evaluation of the physiological state of sperm cells as well as for selection of a suitable method of fertilization in the laboratories of assisted reproduction.
Specificity:
The antibody Hs-14 reacts with VCP (valosin-containing protein) a 220 kDa intra-acrosomal protein.
Antigen
VCP
Clone
Hs-14
Species Reactivity
Mouse, Human
Negative Species
N/A
Isotype
Mouse IgM
Immunogen
Freshly ejaculated human sperms were washed in PBS and extracted in 3% acetic acid, 10% glycerol, 30 mM benzaminidine. The acid extract was dialyzed against 0.2% acetic acid and subsequently used for immunization.
Application
FC (QC tested)
Regulatory status
RUO
Concentration
N/A
Format
FITC
Storage / Stability
Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Storage Buffer
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide