![AffiAB® Anti-Hu CD235a Biotin [JC159]](/web/image/product.product/82616/image_1024/%5BAFG-EXB-1117%5D%20AffiAB%C2%AE%20Anti-Hu%20CD235a%20Biotin%20%5BJC159%5D?unique=d31c23b)
Overview
CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.
Specificity:
The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
Antigen
CD235a
Clone
JC159
Species Reactivity
Rat, Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Membrane preparation from splenic hairy cell leukemia
Application
IHC (P), IHC (F), FC (QC tested)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Biotin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide