![AffiAB® Anti-Hu CD11b Biotin [ICRF44]](/web/image/product.product/82610/image_1024/%5BAFG-EXB-1111%5D%20AffiAB%C2%AE%20Anti-Hu%20CD11b%20Biotin%20%5BICRF44%5D?unique=d31c23b)
Overview
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) ; expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Specificity:
The mouse monoclonal antibody ICRF44 recognizes an extracellular epitope of CD11b (Mac-1alpha), a 165-170 kDa type 1 transmembrane protein mainly expressed on monocytes, granulocytes and NK-cells.
Antigen
CD11b
Clone
ICRF44
Species Reactivity
Human, Non-human primates
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Rheumatoid synovial cells and human monocytes.
Application
FC (QC tested), IP, IHC (F), ICC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Biotin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide