![AffiAB® Anti-Ms MHC Class II Biotin [M5/114]](/web/image/product.product/82600/image_1024/%5BAFG-EXB-1101%5D%20AffiAB%C2%AE%20Anti-Ms%20MHC%20Class%20II%20Biotin%20%5BM5-114%5D?unique=0daa593)
Overview
MHC (major histocompatibility complex) class II molecules are transmembrane glycoproteins expressed on the surface of professional antigen-presenting cells, such as macrophages, dendritic cells and B cells. Before their exposition on the cell surface, the MHC class II molecules react with endocytosed exogenous antigens, which are then presented to the T cells. The antigen-binding grove between MHC class II alpha and beta chain is open at both ends and is 15-24 amino acid residues long.
Specificity:
The rat monoclonal antibody M5/114 reacts with murine MHC class II glycoproteins. It recognizes a shared extracellular determinant on I-Ab, I-Ad, I-Aq, and I-Ed, I-Ek alloantigens, but it does not react with I-Af, I-Ak, I-As. This antibody can inhibit I-A-restricted T cell responses of the H-2b, H-2d, H-2q, H-2u but not H-2f, H-2k, H-2s haplotypes.
Antigen
MHC Class II
Clone
M5/114
Species Reactivity
Mouse
Negative Species
N/A
Isotype
Rat IgG2b
Immunogen
Activated C57BL/6 mouse spleen cells
Application
WB, IHC (P), IP, FC, IHC (F)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Biotin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide