![AffiAB® Anti-HLA Class I Biotin [W6/32]](/web/image/product.product/82580/image_1024/%5BAFG-EXB-1081%5D%20AffiAB%C2%AE%20Anti-HLA%20Class%20I%20Biotin%20%5BW6-32%5D?unique=0daa593)
Overview
HLA-class I major histocompatibility (MHC) antigens are intrinsic membrane glycoproteins expressed on nucleated cells and noncovalently associated with an invariant beta2 microglobulin. They carry foreign determinants important for immune recognition by cytotoxic T cells, thus important for anti-viral and anti-tumour defence. Human HLA-class I antigens are represented by HLA-A, HLA-B and HLA-C molecules.
Specificity:
The antibody W6/32 recognises an extracellular epitope of MHC Class I molecules (MHC Class Ia) that are expressed on the surface of all human nucleated cell types. The antibody W6/32 is a valuable reagent for analysing variations in HLA class I expression in different disease states e.g. liver disease, muscular dystrophy, inflammatory myopathy and other neuromuscular disorders. This antibody W6/32 is also suitable as a positive control for HLA tissue typing and crossmatching.
Antigen
HLA Class I
Clone
W6/32
Species Reactivity
Cow, Non-human primates, Human, Cat
Negative Species
Rabbit
Isotype
Mouse IgG2a
Immunogen
Membrane of human tonsil cells
Application
FC (QC tested), IP, WB, IHC (F), ICC, ELISA
Regulatory status
RUO
Concentration
1 mg/ml
Format
Biotin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide