![AffiAB® Anti-Hu CD8 Biotin [MEM-31]](/web/image/product.product/80001/image_1024/%5BAFG-EXB-1031%5D%20AffiAB%C2%AE%20Anti-Hu%20CD8%20Biotin%20%5BMEM-31%5D?unique=d31c23b)
Overview
The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as alpha/beta heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as alpha/alpha homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 beta level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 alpha-beta but not alpha-alpha dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.
Specificity:
The antibody MEM-31 recognizes a conformationally-dependent extracellular epitope of CD8, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 alpha/alpha homodimer or CD8 alpha/beta heterodimer (each monomer approx. 32-34 kDa) . The antibody does not react with formaldehyde-fixed cells; negative in Western blotting application.
Antigen
CD8
Clone
MEM-31
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG2a
Immunogen
Crude thymus membrane fraction.
Application
FC (QC tested), IP
Regulatory status
RUO
Concentration
1 mg/ml
Format
Biotin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide