![AffiAB® Anti-Hu CD180 Purified Low Endotoxin [G28-8]](/web/image/product.product/79738/image_1024/%5BAFG-EXB-0768%5D%20AffiAB%C2%AE%20Anti-Hu%20CD180%20Purified%20Low%20Endotoxin%20%5BG28-8%5D?unique=d31c23b)
Overview
CD180, also known as RP105 (or Bgp95, LY64) is a type I membrane glycoprotein of Toll-like receptor (TLR) family. Its cytoplasmic tail is short and unlike the TLRs, it lacks the TIR domain. CD180 expression is dependent on the coexpression of its helper molecule, MD-1, and mirrors that of TLR4 on antigen-presenting cells. CD180 regulates recognition of LPS and signaling in B cells, via interacting directly with the TLR4 signaling complex, inhibiting its ability to bind microbial ligands. Ligation of CD180 by monoclonal antibodies leads to B cell activation, upregulation of CD80/CD86, and increase in cell size.
Specificity:
The mouse monoclonal antibody G28-8 recognizes an extracellular epitope of CD180, a 95-105 kDa TLR-like glycoprotein expressed on peripheral blood monocytes and dendritic cells, mantle zone B cells and marginal zone B cells, but very weakly on germinal center B cells.
Antigen
CD180
Clone
G28-8
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1 kappa
Immunogen
Human tonsillar B cells
Application
FC (QC tested), IP, FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Low Endotoxin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4