![AffiAB® Anti-Ms CD86 Purified Low Endotoxin [GL-1]](/web/image/product.product/79734/image_1024/%5BAFG-EXB-0764%5D%20AffiAB%C2%AE%20Anti-Ms%20CD86%20Purified%20Low%20Endotoxin%20%5BGL-1%5D?unique=d31c23b)
Overview
CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CTLA-4 (CD152) . The both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.
Specificity:
The rat monoclonal antibody GL-1 reacts with an extracellular epitope of CD86 (B7-2), a 70-80 kDa type I transmembrane glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.
Antigen
CD86
Clone
GL-1
Species Reactivity
Mouse
Negative Species
N/A
Isotype
Rat IgG2a kappa
Immunogen
LPS-activated CBA/Cs mouse splenic B cells
Application
FC (QC tested), IP, IHC (F), ICC, FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Low Endotoxin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4