![AffiAB® Anti-Ms CD11b Purified Low Endotoxin [M1/70]](/web/image/product.product/79723/image_1024/%5BAFG-EXB-0753%5D%20AffiAB%C2%AE%20Anti-Ms%20CD11b%20Purified%20Low%20Endotoxin%20%5BM1-70%5D?unique=d31c23b)
Overview
CD11b (integrin alphaM subunit) is a 165-170 kDa type I transmembrane glycoprotein that non-covalently associates with integrin beta2 subunit (CD18) ; expression of the CD11b chain on the cell surface requires the presence of the CD18 antigen. CD11b/CD18 integrin (Mac-1, CR3) is highly expressed on NK cells, neutrophils, monocytes and less on macrophages. CD11b/CD18 integrin is implicated in various adhesive interactions of monocytes, macrophages and granulocytes, facilitating their diapedesis, as well as it mediates the uptake of complement coated particles, serving as a receptor for the iC3b fragment of the third complement component.
Specificity:
The rat monoclonal antibody M1/70 detects an extracellular epitope of CD11b (integrin alphaM subunit), a type I transmembrane protein mainly expressed on monocytes/macrophages, granulocytes and NK-cells, which associates with CD18 to form Mac-1 integrin that plays important role in cell-cell interactions.
Antigen
CD11b
Clone
M1/70
Species Reactivity
Mouse, Rabbit, Human, Non-human primates
Negative Species
N/A
Isotype
Rat IgG2b
Immunogen
B10 mouse spleen cells enriched for T cells
Application
FC (QC tested), IP, IHC (F), FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Low Endotoxin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4