![AffiAB® Anti-Hu CD53 Purified Low Endotoxin [MEM-53]](/web/image/product.product/79700/image_1024/%5BAFG-EXB-0730%5D%20AffiAB%C2%AE%20Anti-Hu%20CD53%20Purified%20Low%20Endotoxin%20%5BMEM-53%5D?unique=d31c23b)
Overview
CD53 is a tetraspanin family transmembrane glycoprotein expressed in the lymphoid-myeloid lineage. This molecule has been reported to form complexes with other leukocyte surface proteins such as CD2, CD19, CD21, MHC II, VLA-4 or tetraspanins CD37, CD81 and CD82, thus probably modulating various signaling processes. CD53 is involved in radioresistancy of tumour cells and its triggering has anti-apoptotic effect. In thymus, CD53 is up-regulated in response to positive selection signals during T cell development, and is strongly expressed upon macrophage exposure to bacterial lipopolysaccharide, whereas stimulation of neutrophils results in down-regulation of CD53 expression.
Specificity:
The antibody MEM-53 reacts with an extracellular epitope of CD53, a 32-40 kDa tetraspanin family glycoprotein exclusivelly expressed on leukocytes; it is not present on platelets, red blood cells and non-hematopoietic cells.
The antibody MEM-53 reacts also with deglycosylated molecule (molecular weight of the antigen is reduced by 15 kDa using endoglycosidase F) .
Antigen
CD53
Clone
MEM-53
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Leukocytes of pacient suffering from a LGL-type leukemia.
Application
FC (QC tested), IP, WB, IHC (F), FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Low Endotoxin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4