![AffiAB® Anti-Hu CD18 Purified Low Endotoxin [MEM-48]](/web/image/product.product/79698/image_1024/%5BAFG-EXB-0728%5D%20AffiAB%C2%AE%20Anti-Hu%20CD18%20Purified%20Low%20Endotoxin%20%5BMEM-48%5D?unique=d31c23b)
Overview
CD18, integrin beta2 subunit, forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18) . In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.
Specificity:
The antibody MEM-48 recognizes an extracellular epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen (integrin beta2 subunit; beta2 integrin) . CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.
Antigen
CD18
Clone
MEM-48
Species Reactivity
Human
Negative Species
Dog
Isotype
Mouse IgG1
Immunogen
Leukocytes of a patient suffering from a LGL-type leukemia.
Application
FC (QC tested), IP, WB, IHC (P), IHC (F), FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Low Endotoxin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4