![AffiAB® Anti-Hu CD16 Purified Low Endotoxin [MEM-154]](/web/image/product.product/79697/image_1024/%5BAFG-EXB-0727%5D%20AffiAB%C2%AE%20Anti-Hu%20CD16%20Purified%20Low%20Endotoxin%20%5BMEM-154%5D?unique=d31c23b)
Overview
CD16 (FcgammaRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcgammaRIII is expressed in two forms – FcgammaRIII-A and -B. FcgammaRIII-A is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcgammaRIII-A is associated, moreover, with FcepsilonRI-beta subunit. Besides IgG, FcgammaRIII-A can be triggered also by oligomeric IgE. FcgammaRIII-B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.
Specificity:
The antibody MEM-154 reacts with an extracellular epitope on CD16 antigen that is residing in proximity to FG loop (probably BC or C'E loop) . CD16 is a low affinity receptor for aggregated IgG (FcgammaRIII antigen) . The antibody MEM-154 reacts with CD16+ granulocytes, and it can be used for mapping CD16-158V/F polymorphism on NK cells, as it requires presence of V at amino acid 158.
Antigen
CD16
Clone
MEM-154
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Human granulocytes
Application
FC (QC tested), IP, WB, FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Low Endotoxin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4