![AffiAB® Anti-Hu CD49d Purified Low Endotoxin [9F10]](/web/image/product.product/79689/image_1024/%5BAFG-EXB-0719%5D%20AffiAB%C2%AE%20Anti-Hu%20CD49d%20Purified%20Low%20Endotoxin%20%5B9F10%5D?unique=d31c23b)
Overview
CD49d / integrin alpha 4, unlike other alpha integrins, neither contains an I-domain, nor undergoes disulfide-linked cleavage. It associates with beta 7 chain to form alpha 4 / beta 7 integrin, and with beta 1 chain (CD29) to form VLA-4 integrin. These complexes are important for lymphocyte migration from circulation into tissue (binding VCAM-1) and homing of T cell subsets to Peyer´s patches (binding MadCAM-1), but VLA-4 is also target for invasive bacteria which contain invasin. CD49d is essential for differentiation and migration of hematopoietic stem cells by their adhesion to bone marrow stromal cells, and provides a costimulatory signal to TCR-CD3 complex by inducing phosphorylation of some focal adhesion proteins.
Specificity:
The mouse monoclonal antibody 9F10 recognizes an extracellular epitope of CD49d (alpha 4 integrin), a 145-180 kDa type I transmembrane glycoprotein expressed on B and T cells, monocytes, eosinophils, basophils, NK cells, and dendritic cells, but not platelets.
Antigen
CD49d
Clone
9F10
Species Reactivity
Sheep, Cat, Horse, Dog, Cow, Non-human primates, Human
Negative Species
N/A
Isotype
Mouse IgG1 kappa
Immunogen
N/A
Application
FC (QC tested), IHC (F), FUNC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified Low Endotoxin
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4