![AffiAB® Anti-Hu CD65 Purified [VIM8]](/web/image/product.product/79554/image_1024/%5BAFG-EXB-0584%5D%20AffiAB%C2%AE%20Anti-Hu%20CD65%20Purified%20%5BVIM8%5D?unique=d31c23b)
Overview
CD65 is a fucosylated carbohydrate antigen (ceramide-dodecasaccharide, type II fucoganglioside), which serves as a ligand for CD62E (E-selectin) . Its structure is Gal beta1-4 GlcNAc beta1-3 Gal beta1-4 GlcNAc (3-1 Fuc alpha) beta1-3 ceramide. Unlike CD65s, the CD65 antigen does not contain terminal sialic acid, the rest of their structure is identical. CD65 is expressed on granulocytes and monocytes and participates in cell adhesion. It has been reported as important for extravascular infiltration of acute monocytic leukemia cells.
Specificity:
The mouse monoclonal antibody VIM8 recognizes human CD65, an asialo-fucoganglioside expressed on the surface of peripheral blood granulocytes (highly) and monocytes (weakly) .
Antigen
CD65
Clone
VIM8
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgM
Immunogen
THP-1 cell line
Application
FC (QC tested)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide