![AffiAB® Anti-Basic Cytokeratins Purified [AE3]](/web/image/product.product/79531/image_1024/%5BAFG-EXB-0561%5D%20AffiAB%C2%AE%20Anti-Basic%20Cytokeratins%20Purified%20%5BAE3%5D?unique=06fe5f0)
Overview
Cytokeratins are a subfamily of intermediate filaments and are characterized by remarkable biochemical diversity. Cytokeratins are represented in epithelial tissues by at least 20 different polypeptides, molecular weight between 40 kDa and 68 kDa. The individual cytokeratin polypeptides are designated 1 to 20 and divided into the type I (acidic cytokeratins 9-20) and type II (basic to neutral cytokeratins 1-8) families.
Specificity:
Mouse monoclonal antibody AE3 recognizes all basic type cytokeratins (intracellular antigens), namely K1-K8 (58-67 kDa) . This antibody reacts with all layers of epidermis (basal layer and above) and is an excellent marker for distinguishing carcinomas from non-epithelial tumours.
Antigen
Basic Cytokeratins
Clone
AE3
Species Reactivity
Cow, Rabbit, Mouse, Human, Non-human primates, Chicken, Rat
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Human epidermal keratins
Application
WB, IHC (P), IHC (F)
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide