![AffiAB® Anti-Hu CD230 / Prion Purified [EM-20]](/web/image/product.product/79391/image_1024/%5BAFG-EXB-0421%5D%20AffiAB%C2%AE%20Anti-Hu%20CD230%20-%20Prion%20Purified%20%5BEM-20%5D?unique=d31c23b)
Overview
CD230 / human prion protein (PrP), also known as PRNP, is a ubiquitously expressed GPI-anchored cell surface glycoprotein associating with lipid raft components and functioning as a signaling molecule. CD230 / PrP plays a role in apoptosis in a cell context-dependent manner, is involved in proliferation of epithelial cells and in distribution of junction-associated proteins in human enterocytes. Conversion of this normal cellular prion protein (PrPc) into an abnormal conformer (PrPSc) is the crucial step associated with triggering the pathogenesis of the prion neurodegenerative disorders, such as the Creutzfeld-Jakob disease (CJD) . Whereas PrPc is rich in alpha-helices, the PrPSc form has higher content of beta-sheets and is resistant to proteinase K.
Specificity:
The mouse monoclonal antibody EM-20 recognizes human prion protein (PrP) . Diglycosylated form of PrP has ~ 40 kDa, monoglycosylated form ~ 30 kDa, and nonglycosylated form ~ 19-21 kDa. This antibody is suitable for discrimination between normal cellular prion protein (PrPc) and its conformationally changed form (PrPSc) prion protein.
Antigen
CD230 / Prion
Clone
EM-20
Species Reactivity
Human
Negative Species
N/A
Isotype
Mouse IgG2a
Immunogen
Recombinant human prion protein
Application
WB
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide