![AffiAB® Anti-SOS Purified [SOS-01]](/web/image/product.product/79341/image_1024/%5BAFG-EXB-0371%5D%20AffiAB%C2%AE%20Anti-SOS%20Purified%20%5BSOS-01%5D?unique=1da554d)
Overview
The guanine nucleotide exchange factor Sos (son-of-sevenless) is a complex multidomain protein that activates the small GTPase Ras (H-Ras, K-Ras, N-Ras, but not functionally distinct R-Ras) in response to receptor tyrosine kinase stimulation. Nucleotide exchange activity of Sos is stimulated by allosteric Ras binding. By another (separable) guanine exchange factor domain domain Sos modulates activity of Rac/Rho GTPases. Sos thus integrates signals that affect both gene expression and cytoskeletal reorganization; the Sos-mediated Ras-activation and Rac activation differ in composition and stability of the formed complex.
Specificity:
The antibody SOS-01 reacts with human Sos, an ubiquitously expressed 150 kDa intracellular protein. The epitope sequence is highly conserved and reactivity with multiple species is expected.
Antigen
SOS
Clone
SOS-01
Species Reactivity
Mouse, Human, Non-human primates
Negative Species
N/A
Isotype
Mouse IgG1
Immunogen
Peptide corresponding to amino acids THPSMHRDGPPLLENAHSS of human Sos protein.
Application
WB, ICC
Regulatory status
RUO
Concentration
1 mg/ml
Format
Purified
Storage / Stability
Store at 2-8°C. Do not freeze.
Storage Buffer
Phosphate buffered saline (PBS) , pH 7.4, 15 mM sodium azide