AffiAB® Anti-Orf1 (N. meningitidis) Purified [PAb (489) ]

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CAT# AFG-EXB-0351
Size: 0.1 mg

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Overview

Orf1 (FrpD) is a Neisseria meningitidis outer membrane lipoprotein. Subcellular localization and [3H]palmitic acid labeling in Escherichia coli revealed that FrpD is synthesized with a type II signal peptide for export across the cytoplasmic membrane and is, upon processing to a lipoprotein, sorted to the outer bacterial membrane.

Specificity:

The polyclonal antibody reacts with Orf1 (FrpD), a 28 - 32 kDa Neisseria meningitidis outer membrane lipoprotein. Orf1 is highly conserved in a set of meningococcal strains representative of all serogroups and does not exhibit any similarity to known sequences of other organisms.

Antigen

Orf1 (N. meningitidis)

Clone

PAb (489)

Species Reactivity

Neisseria meningitidis

Negative Species

N/A

Isotype

Rabbit polyclonal

Immunogen

Slices containing purified FrpD250-6xHis-tagged protein were excised from 10% SDS-PAGE gels, grinded, mixed with 1 mg/ml of aluminum hydroxide in PBS, and used to immunize a rabbit.

Application

Regulatory status

RUO

Concentration

1 mg/ml

Format

Purified

Storage / Stability

Store at 2-8°C. Do not freeze.

Storage Buffer

Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide